Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 603531..604111 Replicon chromosome
Accession NZ_CP065386
Organism Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)


Gene name mazF Uniprot ID A0A836MFL2
Locus tag I5Z97_RS03080 Protein ID WP_021114594.1
Coordinates 603531..603875 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U4SAI4
Locus tag I5Z97_RS03085 Protein ID WP_005710601.1
Coordinates 603872..604111 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Z97_RS03070 601453..602844 - 1392 WP_010785980.1 MATE family efflux transporter -
I5Z97_RS03075 602857..603525 - 669 WP_021118711.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
I5Z97_RS03080 603531..603875 - 345 WP_021114594.1 endoribonuclease MazF Toxin
I5Z97_RS03085 603872..604111 - 240 WP_005710601.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I5Z97_RS03090 604297..605172 + 876 WP_196980301.1 putative selenate ABC transporter substrate-binding protein -
I5Z97_RS03095 605284..606033 + 750 WP_075630389.1 phosphonate ABC transporter ATP-binding protein -
I5Z97_RS03100 606023..606808 + 786 WP_075630388.1 ABC transporter permease subunit -
I5Z97_RS03105 606805..607653 + 849 WP_035522402.1 ABC transporter permease subunit -
I5Z97_RS03110 607715..608407 + 693 WP_021118715.1 tellurite resistance TerB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12801.77 Da        Isoelectric Point: 7.3830

>T182512 WP_021114594.1 NZ_CP065386:c603875-603531 [Glaesserella parasuis]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPAIYNRQTGLLICCPLTTKVKGYPFEVDIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Download         Length: 345 bp

>T182512 NZ_CP086480:1077625-1077960 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8782.21 Da        Isoelectric Point: 5.0351

>AT182512 WP_005710601.1 NZ_CP065386:c604111-603872 [Glaesserella parasuis]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Download         Length: 240 bp

>AT182512 NZ_CP086480:1077377-1077625 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836MFL2

Antitoxin

Source ID Structure
AlphaFold DB U4SAI4

References