Detailed information of TA system TA182510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 387032..387671
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6I4QYS7
• Locus tag: I5Z97_RS02000
• Protein ID: WP_021114433.1
• Coordinates: 387336..387671 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I5Z97_RS01995
• Protein ID: WP_164681833.1
• Coordinates: 387032..387328 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS01970	382081..383268	+	1188	WP_016527483.1	elongation factor Tu	-
I5Z97_RS01975	383405..383993	-	589	Protein_372	transposase	-
I5Z97_RS01980	384381..384710	+	330	Protein_373	transposase	-
I5Z97_RS01985	384738..385869	-	1132	Protein_374	IS3 family transposase	-
I5Z97_RS01990	385970..386959	-	990	WP_021116485.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
I5Z97_RS01995	387032..387328	-	297	WP_164681833.1	putative addiction module antidote protein	Antitoxin
I5Z97_RS02000	387336..387671	-	336	WP_021114433.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5Z97_RS02005	387830..388234	+	405	WP_021110905.1	DNA polymerase III subunit psi	-
I5Z97_RS02010	388349..388660	+	312	WP_042906459.1	hypothetical protein	-
I5Z97_RS02015	388754..389692	+	939	WP_196980291.1	hypothetical protein	-
I5Z97_RS02020	389784..390527	+	744	Protein_381	transposase	-
I5Z97_RS02025	390716..390901	-	186	WP_005713975.1	zf-HC2 domain-containing protein	-
I5Z97_RS02030	390898..391470	-	573	WP_035522608.1	sigma-70 family RNA polymerase sigma factor	-
I5Z97_RS02035	391612..391941	+	330	WP_021113605.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	383631..397119	13488
	-	inside	IScluster/Tn	-	-	384381..396081	11700

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-96)

Antitoxin

• Uncharacterized protein HI1420 (9-91)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12079.77 Da
• Isoelectric Point: 9.8168

>T182510 WP_021114433.1 NZ_CP065386:c387671-387336 [Glaesserella parasuis]
MSGIINQIVITDNFSKWLDGLKNPIAKATVARRIKNAVKGNFGDHKALAGTGGLYEMRIATGAGYRIYYAQQGDVIYILL
QGGDKSTQQADIEKAKALWAELKQTGGSNEY

Nucleotide

• Length: 336 bp

>T182510 NZ_CP086480:c796688-796314 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10948.49 Da
• Isoelectric Point: 4.8951

>AT182510 WP_164681833.1 NZ_CP065386:c387328-387032 [Glaesserella parasuis]
MAEKHGLRPFDVAEFLDNEETIQAYLAEVLKEGDQDEFLEALNDVARARGIAELAKQTGLGRESLYKTLSKGSKPRFETI
QKILGAFNLVLIPQIKNS

Nucleotide

• Length: 297 bp

>AT182510 NZ_CP086480:c797146-796778 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A6I4QYS7