Detailed information of TA system TA182509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 294567..296254
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U4SB19
• Locus tag: I5Z97_RS01520
• Protein ID: WP_021111224.1
• Coordinates: 294567..295910 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I5Z97_RS01525
• Protein ID: WP_015940035.1
• Coordinates: 295910..296254 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS01490	289699..290454	-	756	WP_196980283.1	sel1 repeat family protein	-
I5Z97_RS01495	290456..291211	-	756	WP_196980284.1	sel1 repeat family protein	-
I5Z97_RS01500	291421..291613	+	193	Protein_284	hypothetical protein	-
I5Z97_RS01505	291856..293535	+	1680	WP_075630648.1	DNA repair protein RecN	-
I5Z97_RS01510	293615..293842	-	228	Protein_286	transposase	-
I5Z97_RS01515	294016..294453	+	438	WP_075630649.1	ribosomal protein S18-alanine N-acetyltransferase	-
I5Z97_RS01520	294567..295910	+	1344	WP_021111224.1	type II toxin-antitoxin system HipA family toxin	Toxin
I5Z97_RS01525	295910..296254	+	345	WP_015940035.1	helix-turn-helix domain-containing protein	Antitoxin
I5Z97_RS01530	296283..297608	+	1326	WP_021111225.1	ATP-dependent RNA helicase SrmB	-
I5Z97_RS01535	297729..298361	+	633	WP_021110345.1	lipoprotein insertase outer membrane protein LolB	-
I5Z97_RS01540	298363..299208	+	846	WP_021113204.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
I5Z97_RS01545	299236..300186	+	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	293615..293866	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51106.20 Da
• Isoelectric Point: 5.4867

>T182509 WP_021111224.1 NZ_CP065386:294567-295910 [Glaesserella parasuis]
MQIEPLILQAYIADRWQDIAILTFQADYSLNDFSYLHDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFVENEPSRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATRQVWIDNLQQGASSDDFYLVKFPRGKRSVIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIETSHLVETGMFFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T182509 NZ_CP086480:721533-721829 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13225.52 Da
• Isoelectric Point: 10.2782

>AT182509 WP_015940035.1 NZ_CP065386:295910-296254 [Glaesserella parasuis]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT182509 NZ_CP086480:721831-722226 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: U4SB19