Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 5449366..5450874 Replicon chromosome
Accession NZ_CP065374
Organism Pseudomonas aeruginosa strain PAG7

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag I3251_RS25575 Protein ID WP_003119682.1
Coordinates 5449366..5449953 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1C7C4U7
Locus tag I3251_RS25580 Protein ID WP_003091607.1
Coordinates 5450020..5450874 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3251_RS25570 (I3251_25570) 5445621..5449100 - 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
I3251_RS25575 (I3251_25575) 5449366..5449953 + 588 WP_003119682.1 GNAT family protein Toxin
I3251_RS25580 (I3251_25580) 5450020..5450874 + 855 WP_003091607.1 AraC family transcriptional regulator Antitoxin
I3251_RS25585 (I3251_25585) 5450971..5453136 + 2166 WP_003115567.1 TonB-dependent receptor family protein -
I3251_RS25590 (I3251_25590) 5453311..5455185 + 1875 WP_003117607.1 MFS transporter -
I3251_RS25595 (I3251_25595) 5455246..5455455 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T182505 WP_003119682.1 NZ_CP065374:5449366-5449953 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T182505 NZ_CP086476:c26134-25883 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATCACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGAAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31895.20 Da        Isoelectric Point: 7.5295

>AT182505 WP_003091607.1 NZ_CP065374:5450020-5450874 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Download         Length: 855 bp

>AT182505 NZ_CP086476:c25896-25669 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C4U7

References