Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RHH(antitoxin)
Location 3727493..3727998 Replicon chromosome
Accession NZ_CP065374
Organism Pseudomonas aeruginosa strain PAG7

Toxin (Protein)


Gene name parE Uniprot ID A0A069QL22
Locus tag I3251_RS17525 Protein ID WP_003121619.1
Coordinates 3727493..3727774 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID Q9I707
Locus tag I3251_RS17530 Protein ID WP_003112628.1
Coordinates 3727771..3727998 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3251_RS17500 (I3251_17500) 3722744..3724093 + 1350 WP_003119513.1 C4-dicarboxylate transporter DctA -
I3251_RS17505 (I3251_17505) 3724142..3724828 + 687 WP_003083762.1 FadR/GntR family transcriptional regulator -
I3251_RS17510 (I3251_17510) 3724929..3725663 + 735 WP_003117208.1 GntR family transcriptional regulator -
I3251_RS17515 (I3251_17515) 3725843..3726253 + 411 WP_003101225.1 aegerolysin family protein -
I3251_RS17520 (I3251_17520) 3726285..3727193 - 909 WP_003083769.1 LysR family transcriptional regulator -
I3251_RS17525 (I3251_17525) 3727493..3727774 - 282 WP_003121619.1 type II toxin-antitoxin system toxin ParE Toxin
I3251_RS17530 (I3251_17530) 3727771..3727998 - 228 WP_003112628.1 CopG family ribbon-helix-helix protein Antitoxin
I3251_RS17535 (I3251_17535) 3728174..3728794 - 621 WP_003101226.1 hypothetical protein -
I3251_RS17540 (I3251_17540) 3728895..3729395 + 501 WP_003101228.1 LEA type 2 family protein -
I3251_RS17545 (I3251_17545) 3729468..3729809 + 342 WP_003101229.1 zinc ribbon domain-containing protein YjdM -
I3251_RS17550 (I3251_17550) 3729891..3731318 - 1428 WP_003083784.1 GABA permease -
I3251_RS17555 (I3251_17555) 3731487..3732980 - 1494 WP_003083788.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10492.22 Da        Isoelectric Point: 10.0435

>T182504 WP_003121619.1 NZ_CP065374:c3727774-3727493 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDTIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Download         Length: 282 bp

>T182504 NZ_CP086475:9618-9878 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8568.64 Da        Isoelectric Point: 5.5740

>AT182504 WP_003112628.1 NZ_CP065374:c3727998-3727771 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHIEIEKRWGLQ

Download         Length: 228 bp

>AT182504 NZ_CP086475:9868-10119 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A069QL22

Antitoxin

Source ID Structure
PDB 6XRW
AlphaFold DB Q9I707

References