Detailed information of TA system TA182502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 1491116..1492374
• Replicon: chromosome
• Accession: NZ_CP065374
• Organism: Pseudomonas aeruginosa strain PAG7

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: I3251_RS07050
• Protein ID: WP_023084953.1
• Coordinates: 1491116..1491871 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: I3251_RS07055
• Protein ID: WP_003086329.1
• Coordinates: 1492006..1492374 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3251_RS07040 (I3251_07040)	1486289..1488832	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
I3251_RS07045 (I3251_07045)	1488987..1490348	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
I3251_RS07050 (I3251_07050)	1491116..1491871	-	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
I3251_RS07055 (I3251_07055)	1492006..1492374	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
I3251_RS07060 (I3251_07060)	1492447..1493733	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
I3251_RS07065 (I3251_07065)	1493789..1495282	-	1494	WP_282309516.1	aldehyde dehydrogenase family protein	-
I3251_RS07070 (I3251_07070)	1495456..1495932	+	477	WP_009877993.1	elongation factor GreAB	-
I3251_RS07075 (I3251_07075)	1495935..1496210	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T182502 WP_023084953.1 NZ_CP065374:c1491871-1491116 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T182502 NZ_CP086473:c79268-78888 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACAGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT182502 WP_003086329.1 NZ_CP065374:c1492374-1492006 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT182502 NZ_CP086473:c79489-79268 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7