Detailed information of TA system TA182501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 495009..496230
• Replicon: chromosome
• Accession: NZ_CP065374
• Organism: Pseudomonas aeruginosa strain PAG7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: I3251_RS02255
• Protein ID: WP_003102740.1
• Coordinates: 495652..496230 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: I3251_RS02250
• Protein ID: WP_003088112.1
• Coordinates: 495009..495563 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3251_RS02225 (I3251_02225)	490577..491128	+	552	WP_003132721.1	XRE family transcriptional regulator	-
I3251_RS02230 (I3251_02230)	491125..491523	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
I3251_RS02235 (I3251_02235)	491536..491859	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
I3251_RS02240 (I3251_02240)	492190..492651	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
I3251_RS02245 (I3251_02245)	492656..494851	+	2196	WP_003114948.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
I3251_RS02250 (I3251_02250)	495009..495563	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
I3251_RS02255 (I3251_02255)	495652..496230	+	579	WP_003102740.1	HD domain-containing protein	Toxin
I3251_RS02260 (I3251_02260)	496339..497526	-	1188	WP_105248280.1	HlyD family efflux transporter periplasmic adaptor subunit	-
I3251_RS02265 (I3251_02265)	497516..499687	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
I3251_RS02270 (I3251_02270)	499677..500954	-	1278	WP_003122808.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T182501 WP_003102740.1 NZ_CP065374:495652-496230 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T182501 NZ_CP086473:c8675-8373 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT182501 WP_003088112.1 NZ_CP065374:495009-495563 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT182501 NZ_CP086473:c8302-7964 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07