Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 543..1111 Replicon plasmid pVp-20-082A3A
Accession NZ_CP065371
Organism Vibrio parahaemolyticus strain 20-082A3

Toxin (Protein)


Gene name mazF Uniprot ID A0A1Y1BA95
Locus tag I5M77_RS25385 Protein ID WP_023624650.1
Coordinates 543..884 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7Y0S5I5
Locus tag I5M77_RS25390 Protein ID WP_031501799.1
Coordinates 881..1111 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M77_RS25385 543..884 - 342 WP_023624650.1 endoribonuclease MazF Toxin
I5M77_RS25390 881..1111 - 231 WP_031501799.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I5M77_RS25395 1562..2287 + 726 WP_017449237.1 site-specific integrase -
I5M77_RS25400 2282..2665 - 384 Protein_4 recombinase family protein -
I5M77_RS25405 3268..3489 - 222 WP_023624649.1 hypothetical protein -
I5M77_RS25410 3799..4173 + 375 WP_042761155.1 hypothetical protein -
I5M77_RS25415 4734..5738 + 1005 WP_158501040.1 Ig-like domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..121293 121293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-107)

Antitoxin

Antitoxin PemI-like

(1-75)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12159.65 Da        Isoelectric Point: 5.8124

>T182500 WP_023624650.1 NZ_CP065371:c884-543 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Download         Length: 342 bp

>T182500 NZ_CP086472:77203-77619 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8471.81 Da        Isoelectric Point: 5.6330

>AT182500 WP_031501799.1 NZ_CP065371:c1111-881 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNQQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Download         Length: 231 bp

>AT182500 NZ_CP086472:76976-77206 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y1BA95

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y0S5I5

References