Detailed information of TA system TA182499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 2941035..2942383
• Replicon: chromosome
• Accession: NZ_CP065369
• Organism: Vibrio parahaemolyticus strain 20-082A3

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: I5M77_RS14050
• Protein ID: WP_156216669.1
• Coordinates: 2941442..2942383 (+)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A1R4LDG5
• Locus tag: I5M77_RS14045
• Protein ID: WP_000127617.1
• Coordinates: 2941035..2941439 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M77_RS14030	2938856..2940199	+	1344	WP_156216666.1	tyrosine-type recombinase/integrase	-
I5M77_RS14035	2940192..2940575	+	384	WP_156216668.1	hypothetical protein	-
I5M77_RS14040	2940585..2940773	+	189	WP_000342585.1	hypothetical protein	-
I5M77_RS14045	2941035..2941439	+	405	WP_000127617.1	hypothetical protein	Antitoxin
I5M77_RS14050	2941442..2942383	+	942	WP_156216669.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I5M77_RS14055	2942392..2942958	+	567	Protein_2739	SAM-dependent DNA methyltransferase	-
I5M77_RS14060	2943030..2943305	-	276	WP_156216623.1	hypothetical protein	-
I5M77_RS14065	2943609..2943821	+	213	WP_033929743.1	helix-turn-helix domain-containing protein	-
I5M77_RS14070	2943860..2944972	-	1113	WP_156216625.1	hypothetical protein	-
I5M77_RS14075	2944972..2945568	-	597	WP_107111805.1	pancortin-3	-
I5M77_RS14080	2945594..2946310	-	717	WP_196952916.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2932249..2979871	47622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-300)

Antitoxin

• Protein of unknown function DUF6088 (8-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35852.35 Da
• Isoelectric Point: 5.8951

>T182499 WP_156216669.1 NZ_CP065369:2941442-2942383 [Vibrio parahaemolyticus]
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLAELETLSVETHKLVFSGGTALAKSDVKILRMSEDVDIKLIPSQEFVV
LSRGKKKALRKALVEQIEARIGQSGHFAIEDKLVRDEYRYVEYELKYPQQFSQAPCLRPIIKLELMETISLHAIEERSIQ
SLVSELYQQSREVNAFACVSIQATLVEKVLSMLRRTMLVKRDSERNDDATLVRHIYDVHCIDIAEKTTSETLAPLFHTVL
QEDIQRFGNQHAEFVADPVNELNLGLQELESNPLFRQRFDDFVKPMVFNPQPHDFDTCFASFKRIAKSLITTI

Nucleotide

• Length: 942 bp

>T182499 NZ_CP086472:c76196-75891 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15292.58 Da
• Isoelectric Point: 10.3464

>AT182499 WP_000127617.1 NZ_CP065369:2941035-2941439 [Vibrio parahaemolyticus]
MTAVECIYQKIKRSRRYVFERKDFDDVASYDQVGRALRQLVKQGDLIKIGYGLYTKAKLNSLTGKPMPTHPGGTDALMRE
ILKMKGVDFEMDSLSWQSLSGQSTQIPSSVQYSWNPKQFNRKLVVGNRVLNSQN

Nucleotide

• Length: 405 bp

>AT182499 NZ_CP086472:c76416-76198 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1R4LDG5