Detailed information of TA system TA182492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1299873..1300432
• Replicon: chromosome
• Accession: NZ_CP065367
• Organism: Vibrio cyclitrophicus strain ED287

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ITG09_RS21405
• Protein ID: WP_248375168.1
• Coordinates: 1299873..1300187 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B6ERG3
• Locus tag: ITG09_RS21410
• Protein ID: WP_012551865.1
• Coordinates: 1300178..1300432 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG09_RS21385 (ITG09_21360)	1297129..1298136	-	1008	WP_248375166.1	substrate-binding domain-containing protein	-
ITG09_RS21390 (ITG09_21365)	1298281..1298982	-	702	WP_248375167.1	lipoate--protein ligase	-
ITG09_RS21395 (ITG09_21370)	1299164..1299447	+	284	Protein_1084	damage-inducible protein J	-
ITG09_RS21400 (ITG09_21375)	1299447..1299707	+	261	Protein_1085	type II toxin-antitoxin system RelE/ParE family toxin	-
ITG09_RS21405 (ITG09_21380)	1299873..1300187	-	315	WP_248375168.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG09_RS21410 (ITG09_21385)	1300178..1300432	-	255	WP_012551865.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ITG09_RS21415 (ITG09_21390)	1300515..1300592	-	78	WP_248376750.1	DUF3265 domain-containing protein	-
ITG09_RS21420 (ITG09_21395)	1300677..1301183	-	507	WP_248375169.1	YgjV family protein	-
ITG09_RS21425 (ITG09_21400)	1301606..1301887	-	282	WP_248375170.1	hypothetical protein	-
ITG09_RS21430 (ITG09_21405)	1301964..1302422	-	459	WP_248375171.1	hypothetical protein	-
ITG09_RS21435 (ITG09_21410)	1302630..1303133	-	504	WP_248375172.1	hypothetical protein	-
ITG09_RS21440 (ITG09_21415)	1303276..1303806	-	531	WP_248375173.1	GNAT family N-acetyltransferase	-
ITG09_RS21445 (ITG09_21420)	1303932..1305005	-	1074	WP_248375174.1	PIN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	1299447..1308711	9264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-65)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12176.04 Da
• Isoelectric Point: 4.5467

>T182492 WP_248375168.1 NZ_CP065367:c1300187-1299873 [Vibrio cyclitrophicus]
MAVINWTESALLDLDEIAEYIAINNILAAKALVQSVFHRVERLEDFPLSGRSAPELPHLDYREIVLPPCRIFYKVTNDDI
VYILYVMREERELRQFVLNDRGLQ

Nucleotide

• Length: 315 bp

>T182492 NZ_CP086470:3792708-3792986 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9590.14 Da
• Isoelectric Point: 7.3180

>AT182492 WP_012551865.1 NZ_CP065367:c1300432-1300178 [Vibrio cyclitrophicus]
MKVELVTTLKRQATKILSELHASKEPVLITEHGQPSAYLLDVDDYEFMQKRIAILEGIARGEKAISDNRVVSHEVAKEKM
NKWL

Nucleotide

• Length: 255 bp

>AT182492 NZ_CP086470:3792445-3792705 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: B6ERG3