Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4043316..4043931 Replicon chromosome
Accession NZ_CP065358
Organism Kosakonia oryzae strain IAC-BECa90

Toxin (Protein)


Gene name Hha Uniprot ID A0A1C4FBY4
Locus tag I5186_RS19240 Protein ID WP_007373583.1
Coordinates 4043713..4043931 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A6M5CWA9
Locus tag I5186_RS19235 Protein ID WP_007373584.1
Coordinates 4043316..4043690 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5186_RS19225 (I5186_19140) 4038438..4039631 + 1194 WP_227121566.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I5186_RS19230 (I5186_19145) 4039654..4042803 + 3150 WP_064568149.1 multidrug efflux RND transporter permease subunit AcrB -
I5186_RS19235 (I5186_19150) 4043316..4043690 + 375 WP_007373584.1 Hha toxicity modulator TomB Antitoxin
I5186_RS19240 (I5186_19155) 4043713..4043931 + 219 WP_007373583.1 HHA domain-containing protein Toxin
I5186_RS19245 (I5186_19160) 4043997..4044557 + 561 WP_064568150.1 maltose O-acetyltransferase -
I5186_RS19250 (I5186_19165) 4044658..4045137 + 480 WP_227121567.1 YlaC family protein -
I5186_RS19255 (I5186_19170) 4045103..4046548 - 1446 WP_227121568.1 PLP-dependent aminotransferase family protein -
I5186_RS19260 (I5186_19175) 4046646..4047347 + 702 WP_227121569.1 GNAT family protein -
I5186_RS19265 (I5186_19180) 4047344..4047484 - 141 WP_007373577.1 type B 50S ribosomal protein L36 -
I5186_RS19270 (I5186_19185) 4047488..4047748 - 261 WP_043954585.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8637.05 Da        Isoelectric Point: 7.9893

>T182484 WP_007373583.1 NZ_CP065358:4043713-4043931 [Kosakonia oryzae]
MSEKPLTKVDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T182484 NZ_CP086470:c1920890-1920513 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14567.42 Da        Isoelectric Point: 5.8339

>AT182484 WP_007373584.1 NZ_CP065358:4043316-4043690 [Kosakonia oryzae]
MDEYSPKRHDIAQLKFLCETLYHDCLVNLEESHHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTHDLQKWRKTGNKLFRCFVNVSKANPVSHSC

Download         Length: 375 bp

>AT182484 NZ_CP086470:c1921348-1920980 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C4FBY4

Antitoxin

Source ID Structure
AlphaFold DB A0A6M5CWA9

References