Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2064143..2064657 Replicon chromosome
Accession NZ_CP065355
Organism Staphylococcus aureus strain JW31330_O1_dhc

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag I5046_RS10065 Protein ID WP_000581792.1
Coordinates 2064143..2064352 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A0D1GBN9
Locus tag I5046_RS10070 Protein ID WP_000138934.1
Coordinates 2064364..2064657 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5046_RS10045 (I5046_10045) 2059651..2061009 - 1359 WP_000611467.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
I5046_RS10050 (I5046_10050) 2061024..2062094 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
I5046_RS10055 (I5046_10055) 2062412..2063614 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
I5046_RS10060 (I5046_10060) 2063878..2064015 - 138 WP_000828354.1 Lmo0850 family protein -
I5046_RS10065 (I5046_10065) 2064143..2064352 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
I5046_RS10070 (I5046_10070) 2064364..2064657 - 294 WP_000138934.1 copper-sensing transcriptional repressor CsoR Antitoxin
I5046_RS10075 (I5046_10075) 2064823..2066307 + 1485 WP_000571549.1 cardiolipin synthase -
I5046_RS10080 (I5046_10080) 2066335..2066982 + 648 WP_001187619.1 HD domain-containing protein -
I5046_RS10085 (I5046_10085) 2067896..2068768 - 873 WP_000725802.1 membrane protein insertase YidC -
I5046_RS10090 (I5046_10090) 2068854..2069495 - 642 WP_000483165.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(3-95)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T182470 WP_000581792.1 NZ_CP065355:c2064352-2064143 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T182470 NZ_CP086467:c4797419-4797078 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11185.77 Da        Isoelectric Point: 6.2243

>AT182470 WP_000138934.1 NZ_CP065355:c2064657-2064364 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT182470 NZ_CP086467:c4797781-4797440 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1GBN9

References