Detailed information of TA system TA182469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2044399..2044928
• Replicon: chromosome
• Accession: NZ_CP065355
• Organism: Staphylococcus aureus strain JW31330_O1_dhc

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I5046_RS09975
• Protein ID: WP_000621175.1
• Coordinates: 2044399..2044761 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I5046_RS09980
• Protein ID: WP_000948331.1
• Coordinates: 2044758..2044928 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5046_RS09955 (I5046_09955)	2041377..2042147	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
I5046_RS09960 (I5046_09960)	2042122..2042601	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
I5046_RS09965 (I5046_09965)	2042603..2042929	-	327	WP_001052491.1	anti-sigma factor antagonist	-
I5046_RS09970 (I5046_09970)	2043048..2044049	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I5046_RS09975 (I5046_09975)	2044399..2044761	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I5046_RS09980 (I5046_09980)	2044758..2044928	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I5046_RS09985 (I5046_09985)	2045013..2046161	-	1149	WP_196957490.1	alanine racemase	-
I5046_RS09990 (I5046_09990)	2046227..2046586	-	360	WP_000581197.1	holo-ACP synthase	-
I5046_RS09995 (I5046_09995)	2046590..2047081	-	492	WP_001205916.1	PH domain-containing protein	-
I5046_RS10000 (I5046_10000)	2047068..2048651	-	1584	WP_001294642.1	PH domain-containing protein	-
I5046_RS10005 (I5046_10005)	2048644..2049123	-	480	WP_001287083.1	hypothetical protein	-
I5046_RS10010 (I5046_10010)	2049332..2049892	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T182469 WP_000621175.1 NZ_CP065355:c2044761-2044399 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T182469 NZ_CP086467:c4766947-4766414 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT182469 WP_000948331.1 NZ_CP065355:c2044928-2044758 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT182469 NZ_CP086467:c4767224-4766958 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1