Detailed information of TA system TA182463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 811552..812029
• Replicon: chromosome
• Accession: NZ_CP065355
• Organism: Staphylococcus aureus strain JW31330_O1_dhc

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: I5046_RS03795
• Protein ID: WP_000052076.1
• Coordinates: 811552..811740 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: I5046_RS03800
• Protein ID: WP_001059690.1
• Coordinates: 811769..812029 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5046_RS03765 (I5046_03765)	807539..808060	+	522	WP_000824483.1	hypothetical protein	-
I5046_RS03770 (I5046_03770)	808401..809087	+	687	WP_001813074.1	thermonuclease family protein	-
I5046_RS03775 (I5046_03775)	809444..809644	+	201	WP_001059082.1	cold-shock protein	-
I5046_RS03780 (I5046_03780)	810142..810360	-	219	WP_000005880.1	hypothetical protein	-
I5046_RS03785 (I5046_03785)	810423..810707	-	285	WP_001004401.1	hypothetical protein	-
I5046_RS03790 (I5046_03790)	810795..811364	-	570	WP_001106649.1	lipoprotein N-acylation protein LnsA	-
I5046_RS03795 (I5046_03795)	811552..811740	-	189	WP_000052076.1	hypothetical protein	Toxin
I5046_RS03800 (I5046_03800)	811769..812029	-	261	WP_001059690.1	hypothetical protein	Antitoxin
I5046_RS03805 (I5046_03805)	812370..812573	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
I5046_RS03810 (I5046_03810)	812570..812806	-	237	WP_000368687.1	hypothetical protein	-
I5046_RS03815 (I5046_03815)	813031..813612	+	582	WP_001140696.1	histidine phosphatase family protein	-
I5046_RS03820 (I5046_03820)	813685..814302	-	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
I5046_RS03825 (I5046_03825)	814458..814952	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
I5046_RS03830 (I5046_03830)	815351..815773	-	423	WP_000974455.1	organic hydroperoxide resistance protein	-
I5046_RS03835 (I5046_03835)	815921..816637	+	717	WP_000150029.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T182463 WP_000052076.1 NZ_CP065355:c811740-811552 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T182463 NZ_CP086467:360636-360983 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT182463 WP_001059690.1 NZ_CP065355:c812029-811769 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT182463 NZ_CP086467:360983-361282 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56