Detailed information of TA system TA182458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2053708..2054237
• Replicon: chromosome
• Accession: NZ_CP065354
• Organism: Staphylococcus aureus strain JW_comp_novo

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I5044_RS09995
• Protein ID: WP_000621175.1
• Coordinates: 2053708..2054070 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I5044_RS10000
• Protein ID: WP_000948331.1
• Coordinates: 2054067..2054237 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5044_RS09975 (I5044_09975)	2050686..2051456	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
I5044_RS09980 (I5044_09980)	2051431..2051910	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
I5044_RS09985 (I5044_09985)	2051912..2052238	-	327	WP_001052491.1	anti-sigma factor antagonist	-
I5044_RS09990 (I5044_09990)	2052357..2053358	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I5044_RS09995 (I5044_09995)	2053708..2054070	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I5044_RS10000 (I5044_10000)	2054067..2054237	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I5044_RS10005 (I5044_10005)	2054322..2055470	-	1149	WP_001281148.1	alanine racemase	-
I5044_RS10010 (I5044_10010)	2055536..2055895	-	360	WP_000581197.1	holo-ACP synthase	-
I5044_RS10015 (I5044_10015)	2055899..2056390	-	492	WP_001205916.1	PH domain-containing protein	-
I5044_RS10020 (I5044_10020)	2056377..2057960	-	1584	WP_001294642.1	PH domain-containing protein	-
I5044_RS10025 (I5044_10025)	2057953..2058432	-	480	WP_001287083.1	hypothetical protein	-
I5044_RS10030 (I5044_10030)	2058641..2059201	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T182458 WP_000621175.1 NZ_CP065354:c2054070-2053708 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T182458 NZ_CP086464:c4890078-4889794 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT182458 WP_000948331.1 NZ_CP065354:c2054237-2054067 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT182458 NZ_CP086464:c4890310-4890068 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1