Detailed information of TA system TA182453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 821187..821664
• Replicon: chromosome
• Accession: NZ_CP065354
• Organism: Staphylococcus aureus strain JW_comp_novo

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: I5044_RS03845
• Protein ID: WP_000052076.1
• Coordinates: 821187..821375 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: I5044_RS03850
• Protein ID: WP_001059690.1
• Coordinates: 821404..821664 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5044_RS03815 (I5044_03815)	817174..817695	+	522	WP_000824483.1	hypothetical protein	-
I5044_RS03820 (I5044_03820)	818036..818722	+	687	WP_001813074.1	thermonuclease family protein	-
I5044_RS03825 (I5044_03825)	819079..819279	+	201	WP_001059082.1	cold-shock protein	-
I5044_RS03830 (I5044_03830)	819777..819995	-	219	WP_000005880.1	hypothetical protein	-
I5044_RS03835 (I5044_03835)	820058..820342	-	285	WP_001004401.1	hypothetical protein	-
I5044_RS03840 (I5044_03840)	820430..820999	-	570	WP_001106649.1	lipoprotein N-acylation protein LnsA	-
I5044_RS03845 (I5044_03845)	821187..821375	-	189	WP_000052076.1	hypothetical protein	Toxin
I5044_RS03850 (I5044_03850)	821404..821664	-	261	WP_001059690.1	hypothetical protein	Antitoxin
I5044_RS03855 (I5044_03855)	822005..822208	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
I5044_RS03860 (I5044_03860)	822205..822441	-	237	WP_000368687.1	hypothetical protein	-
I5044_RS03865 (I5044_03865)	822666..823247	+	582	WP_001140696.1	histidine phosphatase family protein	-
I5044_RS03870 (I5044_03870)	823320..823937	-	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
I5044_RS03875 (I5044_03875)	824093..824587	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
I5044_RS03880 (I5044_03880)	824986..825408	-	423	WP_000974455.1	organic hydroperoxide resistance protein	-
I5044_RS03885 (I5044_03885)	825556..826272	+	717	WP_000150029.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	813611..824587	10976
	-	flank	IS/Tn	-	-	816115..816879	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T182453 WP_000052076.1 NZ_CP065354:c821375-821187 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T182453 NZ_CP086464:c2555445-2555128 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT182453 WP_001059690.1 NZ_CP065354:c821664-821404 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT182453 NZ_CP086464:c2555052-2554756 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56