Detailed information of TA system TA182447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2074834..2075348
• Replicon: chromosome
• Accession: NZ_CP065353
• Organism: Staphylococcus aureus strain AR39_O1_merpol

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: I5043_RS10055
• Protein ID: WP_000581796.1
• Coordinates: 2074834..2075043 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: I5043_RS10060
• Protein ID: WP_000138932.1
• Coordinates: 2075055..2075348 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5043_RS10035 (I5043_10035)	2070339..2071697	-	1359	WP_000611455.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
I5043_RS10040 (I5043_10040)	2071712..2072782	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
I5043_RS10045 (I5043_10045)	2073100..2074302	+	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
I5043_RS10050 (I5043_10050)	2074569..2074706	-	138	WP_000828354.1	Lmo0850 family protein	-
I5043_RS10055 (I5043_10055)	2074834..2075043	-	210	WP_000581796.1	heavy metal-associated domain-containing protein	Toxin
I5043_RS10060 (I5043_10060)	2075055..2075348	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
I5043_RS10065 (I5043_10065)	2075514..2076998	+	1485	WP_000571549.1	cardiolipin synthase	-
I5043_RS10070 (I5043_10070)	2077026..2077673	+	648	WP_065315756.1	HD domain-containing protein	-
I5043_RS10075 (I5043_10075)	2078410..2079282	-	873	WP_000725802.1	membrane protein insertase YidC	-
I5043_RS10080 (I5043_10080)	2079368..2080009	-	642	WP_000483160.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7859.77 Da
• Isoelectric Point: 4.1008

>T182447 WP_000581796.1 NZ_CP065353:c2075043-2074834 [Staphylococcus aureus]
MIHQNTIYTSGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T182447 NZ_CP086461:c5374615-5374232 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT182447 WP_000138932.1 NZ_CP065353:c2075348-2075055 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT182447 NZ_CP086461:c5374857-5374615 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: W8UW13