Detailed information of TA system TA182442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 812800..813277
• Replicon: chromosome
• Accession: NZ_CP065353
• Organism: Staphylococcus aureus strain AR39_O1_merpol

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: I5043_RS03780
• Protein ID: WP_000052076.1
• Coordinates: 812800..812988 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: I5043_RS03785
• Protein ID: WP_001059690.1
• Coordinates: 813017..813277 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5043_RS03750 (I5043_03750)	808788..809309	+	522	WP_000824478.1	hypothetical protein	-
I5043_RS03755 (I5043_03755)	809650..810336	+	687	WP_001641381.1	thermonuclease family protein	-
I5043_RS03760 (I5043_03760)	810693..810893	+	201	WP_001059082.1	cold-shock protein	-
I5043_RS03765 (I5043_03765)	811391..811609	-	219	WP_000005880.1	hypothetical protein	-
I5043_RS03770 (I5043_03770)	811671..811955	-	285	WP_001004402.1	hypothetical protein	-
I5043_RS03775 (I5043_03775)	812043..812612	-	570	WP_001106650.1	lipoprotein N-acylation protein LnsA	-
I5043_RS03780 (I5043_03780)	812800..812988	-	189	WP_000052076.1	hypothetical protein	Toxin
I5043_RS03785 (I5043_03785)	813017..813277	-	261	WP_001059690.1	hypothetical protein	Antitoxin
I5043_RS03790 (I5043_03790)	813618..813821	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
I5043_RS03795 (I5043_03795)	813818..814054	-	237	WP_000368687.1	hypothetical protein	-
I5043_RS03800 (I5043_03800)	814279..814860	+	582	WP_001140696.1	histidine phosphatase family protein	-
I5043_RS03805 (I5043_03805)	814933..815550	-	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
I5043_RS03810 (I5043_03810)	815706..816200	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
I5043_RS03815 (I5043_03815)	816599..817021	-	423	WP_000974462.1	organic hydroperoxide resistance protein	-
I5043_RS03820 (I5043_03820)	817169..817885	+	717	WP_000150023.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T182442 WP_000052076.1 NZ_CP065353:c812988-812800 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T182442 NZ_CP086461:4125112-4125330 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT182442 WP_001059690.1 NZ_CP065353:c813277-813017 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT182442 NZ_CP086461:4124711-4125085 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56