Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5277990..5278615 Replicon chromosome
Accession NZ_CP065351
Organism Klebsiella pneumoniae strain ZG2017CW4-3-1-2N

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5Q74_RS25790 Protein ID WP_002882817.1
Coordinates 5277990..5278373 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5Q74_RS25795 Protein ID WP_004150355.1
Coordinates 5278373..5278615 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q74_RS25775 5275356..5276258 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I5Q74_RS25780 5276255..5276890 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5Q74_RS25785 5276887..5277816 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5Q74_RS25790 5277990..5278373 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5Q74_RS25795 5278373..5278615 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5Q74_RS25800 5278820..5279737 + 918 WP_009484979.1 alpha/beta hydrolase -
I5Q74_RS25805 5279751..5280692 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5Q74_RS25810 5280737..5281174 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5Q74_RS25815 5281171..5282031 - 861 WP_023280688.1 virulence factor BrkB family protein -
I5Q74_RS25820 5282025..5282624 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182440 WP_002882817.1 NZ_CP065351:c5278373-5277990 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182440 NZ_CP086461:c2555446-2555129 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182440 WP_004150355.1 NZ_CP065351:c5278615-5278373 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182440 NZ_CP086461:c2555053-2554757 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References