Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4060866..4061485 Replicon chromosome
Accession NZ_CP065351
Organism Klebsiella pneumoniae strain ZG2017CW4-3-1-2N

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I5Q74_RS20000 Protein ID WP_002892050.1
Coordinates 4061267..4061485 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I5Q74_RS19995 Protein ID WP_002892066.1
Coordinates 4060866..4061240 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q74_RS19985 4056018..4057211 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I5Q74_RS19990 4057234..4060380 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I5Q74_RS19995 4060866..4061240 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I5Q74_RS20000 4061267..4061485 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I5Q74_RS20005 4061644..4062210 + 567 WP_002892030.1 maltose O-acetyltransferase -
I5Q74_RS20010 4062182..4062322 - 141 WP_004147370.1 hypothetical protein -
I5Q74_RS20015 4062343..4062813 + 471 WP_002892026.1 YlaC family protein -
I5Q74_RS20020 4062788..4064239 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I5Q74_RS20025 4064340..4065038 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
I5Q74_RS20030 4065035..4065175 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I5Q74_RS20035 4065175..4065438 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182437 WP_002892050.1 NZ_CP065351:4061267-4061485 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182437 NZ_CP086461:360636-360983 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182437 WP_002892066.1 NZ_CP065351:4060866-4061240 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182437 NZ_CP086461:360983-361282 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References