Detailed information of TA system TA182436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2655493..2657081
• Replicon: chromosome
• Accession: NZ_CP065351
• Organism: Klebsiella pneumoniae strain ZG2017CW4-3-1-2N

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: J2LTC9
• Locus tag: I5Q74_RS13015
• Protein ID: WP_004148372.1
• Coordinates: 2655493..2656815 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I5Q74_RS13020
• Protein ID: WP_004176198.1
• Coordinates: 2656815..2657081 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Q74_RS13000	2650549..2653050	+	2502	WP_040025883.1	fimbrial biogenesis outer membrane usher protein	-
I5Q74_RS13005	2653038..2654042	+	1005	WP_040025882.1	type 1 fimbrial protein	-
I5Q74_RS13010	2654184..2655476	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
I5Q74_RS13015	2655493..2656815	-	1323	WP_004148372.1	type II toxin-antitoxin system HipA family toxin	Toxin
I5Q74_RS13020	2656815..2657081	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I5Q74_RS13025	2657467..2657793	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
I5Q74_RS13030	2657790..2658290	-	501	WP_020804988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I5Q74_RS13035	2658268..2659647	-	1380	WP_004199850.1	purine permease	-
I5Q74_RS13040	2659993..2661147	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
I5Q74_RS13045	2661128..2662051	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2453338..2692685	239347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49127.45 Da
• Isoelectric Point: 7.5357

>T182436 WP_004148372.1 NZ_CP065351:c2656815-2655493 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSLRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T182436 NZ_CP086461:334675-335043 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT182436 WP_004176198.1 NZ_CP065351:c2657081-2656815 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT182436 NZ_CP086461:334457-334678 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: J2LTC9