Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1498391..1499127 Replicon chromosome
Accession NZ_CP065351
Organism Klebsiella pneumoniae strain ZG2017CW4-3-1-2N

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag I5Q74_RS07395 Protein ID WP_032433360.1
Coordinates 1498645..1499127 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I5Q74_RS07390 Protein ID WP_003026799.1
Coordinates 1498391..1498657 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q74_RS07365 1494038..1495177 + 1140 WP_040025615.1 mannitol dehydrogenase -
I5Q74_RS07370 1495206..1495868 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
I5Q74_RS07375 1495852..1496856 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
I5Q74_RS07380 1496874..1497506 + 633 WP_040025617.1 3-hexulose-6-phosphate synthase -
I5Q74_RS07385 1497516..1498079 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
I5Q74_RS07390 1498391..1498657 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I5Q74_RS07395 1498645..1499127 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
I5Q74_RS07400 1499488..1500087 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
I5Q74_RS07405 1500300..1501244 - 945 WP_077256325.1 fimbrial protein -
I5Q74_RS07410 1501256..1501834 - 579 WP_032432061.1 type 1 fimbrial protein -
I5Q74_RS07415 1501838..1502578 - 741 WP_048978466.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1468615..1510072 41457


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T182427 WP_032433360.1 NZ_CP065351:1498645-1499127 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T182427 NZ_CP086458:c2555442-2555125 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT182427 WP_003026799.1 NZ_CP065351:1498391-1498657 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT182427 NZ_CP086458:c2555049-2554753 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References