Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1273825..1274444 Replicon chromosome
Accession NZ_CP065347
Organism Klebsiella pneumoniae strain ZG2017CW4-4-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I5I21_RS06095 Protein ID WP_002892050.1
Coordinates 1273825..1274043 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I5I21_RS06100 Protein ID WP_002892066.1
Coordinates 1274070..1274444 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5I21_RS06060 1269872..1270135 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
I5I21_RS06065 1270135..1270275 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I5I21_RS06070 1270272..1270970 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
I5I21_RS06075 1271071..1272522 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I5I21_RS06080 1272497..1272967 - 471 WP_002892026.1 YlaC family protein -
I5I21_RS06085 1272988..1273128 + 141 WP_004147370.1 hypothetical protein -
I5I21_RS06090 1273100..1273666 - 567 WP_002892030.1 maltose O-acetyltransferase -
I5I21_RS06095 1273825..1274043 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I5I21_RS06100 1274070..1274444 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I5I21_RS06105 1274930..1278076 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I5I21_RS06110 1278099..1279292 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182409 WP_002892050.1 NZ_CP065347:c1274043-1273825 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182409 NZ_CP086450:c21253-20837 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182409 WP_002892066.1 NZ_CP065347:c1274444-1274070 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182409 NZ_CP086450:c21480-21250 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References