Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59744..60269 Replicon plasmid unnamed2
Accession NZ_CP065345
Organism Klebsiella pneumoniae strain ZG2017CW4-4-1-2

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag I5Q75_RS27185 Protein ID WP_006788214.1
Coordinates 59964..60269 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A379YN57
Locus tag I5Q75_RS27180 Protein ID WP_063445614.1
Coordinates 59744..59962 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q75_RS27160 54900..55679 + 780 WP_013023780.1 hypothetical protein -
I5Q75_RS27165 55886..57478 + 1593 WP_015344964.1 hypothetical protein -
I5Q75_RS27170 57512..58639 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
I5Q75_RS27175 58636..58926 + 291 WP_013023783.1 hypothetical protein -
I5Q75_RS27180 59744..59962 + 219 WP_063445614.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I5Q75_RS27185 59964..60269 + 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
I5Q75_RS27190 60451..61467 + 1017 WP_016946794.1 hypothetical protein -
I5Q75_RS27195 61665..62459 + 795 WP_016240356.1 site-specific integrase -
I5Q75_RS27200 62875..63054 - 180 WP_006788217.1 hypothetical protein -
I5Q75_RS27205 63174..63800 - 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..64300 64300


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T182404 WP_006788214.1 NZ_CP065345:59964-60269 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T182404 NZ_CP086447:c4788058-4787525 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTTGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8307.23 Da        Isoelectric Point: 4.5203

>AT182404 WP_063445614.1 NZ_CP065345:59744-59962 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT182404 NZ_CP086447:c4788335-4788069 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A379YN57

References