Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1273817..1274436 Replicon chromosome
Accession NZ_CP065343
Organism Klebsiella pneumoniae strain ZG2017CW4-4-1-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I5Q75_RS06095 Protein ID WP_002892050.1
Coordinates 1273817..1274035 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I5Q75_RS06100 Protein ID WP_002892066.1
Coordinates 1274062..1274436 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q75_RS06060 1269864..1270127 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
I5Q75_RS06065 1270127..1270267 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I5Q75_RS06070 1270264..1270962 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
I5Q75_RS06075 1271063..1272514 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I5Q75_RS06080 1272489..1272959 - 471 WP_002892026.1 YlaC family protein -
I5Q75_RS06085 1272980..1273120 + 141 WP_004147370.1 hypothetical protein -
I5Q75_RS06090 1273092..1273658 - 567 WP_002892030.1 maltose O-acetyltransferase -
I5Q75_RS06095 1273817..1274035 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I5Q75_RS06100 1274062..1274436 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I5Q75_RS06105 1274922..1278068 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I5Q75_RS06110 1278091..1279284 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182390 WP_002892050.1 NZ_CP065343:c1274035-1273817 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182390 NZ_CP086439:4192324-4192542 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182390 WP_002892066.1 NZ_CP065343:c1274436-1274062 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182390 NZ_CP086439:4191923-4192297 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References