Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 78797..79422 Replicon chromosome
Accession NZ_CP065343
Organism Klebsiella pneumoniae strain ZG2017CW4-4-1-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5Q75_RS00375 Protein ID WP_002882817.1
Coordinates 79039..79422 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5Q75_RS00370 Protein ID WP_004150355.1
Coordinates 78797..79039 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q75_RS00345 74788..75387 + 600 WP_004151865.1 glucose-1-phosphatase -
I5Q75_RS00350 75381..76241 + 861 WP_002882807.1 virulence factor BrkB family protein -
I5Q75_RS00355 76238..76675 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5Q75_RS00360 76720..77661 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5Q75_RS00365 77675..78592 - 918 WP_002882812.1 alpha/beta hydrolase -
I5Q75_RS00370 78797..79039 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5Q75_RS00375 79039..79422 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5Q75_RS00380 79596..80525 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5Q75_RS00385 80522..81157 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5Q75_RS00390 81154..82056 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182386 WP_002882817.1 NZ_CP065343:79039-79422 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182386 NZ_CP086439:1841824-1842156 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182386 WP_004150355.1 NZ_CP065343:78797-79039 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182386 NZ_CP086439:1841566-1841823 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References