Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7792..8317 Replicon plasmid unnamed
Accession NZ_CP065342
Organism Klebsiella pneumoniae strain ZG2017CW 1-1-1

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag I5Q76_RS26200 Protein ID WP_008322213.1
Coordinates 7792..8097 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag I5Q76_RS26205 Protein ID WP_032934863.1
Coordinates 8099..8317 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q76_RS26170 2893..3045 + 153 Protein_2 IS5/IS1182 family transposase -
I5Q76_RS26175 3117..4237 + 1121 WP_089046468.1 IS3 family transposase -
I5Q76_RS26180 4266..5012 + 747 Protein_4 IS5 family transposase -
I5Q76_RS26185 5174..6496 + 1323 WP_016479957.1 GntP family transporter -
I5Q76_RS26190 6695..7486 - 792 WP_008322208.1 site-specific integrase -
I5Q76_RS26195 7476..7790 - 315 WP_008322211.1 hypothetical protein -
I5Q76_RS26200 7792..8097 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
I5Q76_RS26205 8099..8317 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I5Q76_RS26210 9179..9876 - 698 Protein_10 IS1 family transposase -
I5Q76_RS26215 10089..12671 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..107590 107590
- inside IScluster/Tn - - 2929..24917 21988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T182385 WP_008322213.1 NZ_CP065342:c8097-7792 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T182385 NZ_CP086439:799463-799873 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT182385 WP_032934863.1 NZ_CP065342:c8317-8099 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT182385 NZ_CP086439:799216-799482 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References