Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1498395..1499131 Replicon chromosome
Accession NZ_CP065341
Organism Klebsiella pneumoniae strain ZG2017CW 1-1-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag I5Q76_RS07395 Protein ID WP_032433360.1
Coordinates 1498649..1499131 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I5Q76_RS07390 Protein ID WP_003026799.1
Coordinates 1498395..1498661 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5Q76_RS07365 1494042..1495181 + 1140 WP_040025615.1 mannitol dehydrogenase -
I5Q76_RS07370 1495210..1495872 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
I5Q76_RS07375 1495856..1496860 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
I5Q76_RS07380 1496878..1497510 + 633 WP_040025617.1 3-hexulose-6-phosphate synthase -
I5Q76_RS07385 1497520..1498083 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
I5Q76_RS07390 1498395..1498661 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I5Q76_RS07395 1498649..1499131 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
I5Q76_RS07400 1499492..1500091 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
I5Q76_RS07405 1500304..1501248 - 945 WP_077256325.1 fimbrial protein -
I5Q76_RS07410 1501260..1501838 - 579 WP_032432061.1 type 1 fimbrial protein -
I5Q76_RS07415 1501842..1502582 - 741 WP_048978466.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1468619..1510076 41457


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T182371 WP_032433360.1 NZ_CP065341:1498649-1499131 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T182371 NZ_CP086431:343191-343559 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT182371 WP_003026799.1 NZ_CP065341:1498395-1498661 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT182371 NZ_CP086431:342973-343194 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References