Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2877193..2877850 Replicon chromosome
Accession NZ_CP065338
Organism Klebsiella michiganensis strain LDS17

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag I4W82_RS13490 Protein ID WP_004854060.1
Coordinates 2877440..2877850 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag I4W82_RS13485 Protein ID WP_004124953.1
Coordinates 2877193..2877459 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4W82_RS13470 (I4W82_13465) 2872427..2872852 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
I4W82_RS13475 (I4W82_13470) 2872973..2875771 - 2799 WP_209420364.1 transcriptional regulator DagR -
I4W82_RS13480 (I4W82_13475) 2875965..2876948 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
I4W82_RS13485 (I4W82_13480) 2877193..2877459 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
I4W82_RS13490 (I4W82_13485) 2877440..2877850 + 411 WP_004854060.1 protein YgfX Toxin
I4W82_RS13495 (I4W82_13490) 2877859..2878380 - 522 WP_014226792.1 flavodoxin FldB -
I4W82_RS13500 (I4W82_13495) 2878502..2879398 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
I4W82_RS13505 (I4W82_13500) 2879421..2880134 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I4W82_RS13510 (I4W82_13505) 2880140..2881873 + 1734 WP_032751182.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T182359 WP_004854060.1 NZ_CP065338:2877440-2877850 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T182359 NZ_CP086423:799464-799874 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT182359 WP_004124953.1 NZ_CP065338:2877193-2877459 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT182359 NZ_CP086423:799217-799483 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References