Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2778620..2779395 Replicon chromosome
Accession NZ_CP065338
Organism Klebsiella michiganensis strain LDS17

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2J5Q8N8
Locus tag I4W82_RS12995 Protein ID WP_049101740.1
Coordinates 2778910..2779395 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A2J5Q8I3
Locus tag I4W82_RS12990 Protein ID WP_049101739.1
Coordinates 2778620..2778913 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4W82_RS12975 (I4W82_12970) 2773776..2774921 - 1146 WP_004854188.1 PLP-dependent aspartate aminotransferase family protein -
I4W82_RS12980 (I4W82_12975) 2774932..2776302 - 1371 WP_025105933.1 cystathionine beta-synthase -
I4W82_RS12985 (I4W82_12980) 2776848..2778236 + 1389 WP_032751266.1 DASS family sodium-coupled anion symporter -
I4W82_RS12990 (I4W82_12985) 2778620..2778913 + 294 WP_049101739.1 DUF1778 domain-containing protein Antitoxin
I4W82_RS12995 (I4W82_12990) 2778910..2779395 + 486 WP_049101740.1 GNAT family N-acetyltransferase Toxin
I4W82_RS13000 (I4W82_12995) 2780108..2780359 - 252 WP_071846062.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-143)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17553.48 Da        Isoelectric Point: 8.1567

>T182358 WP_049101740.1 NZ_CP065338:2778910-2779395 [Klebsiella michiganensis]
MISTPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQLSGASRTFVCCHDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDRSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLVN
A

Download         Length: 486 bp

>T182358 NZ_CP086423:343192-343560 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10902.55 Da        Isoelectric Point: 9.8590

>AT182358 WP_049101739.1 NZ_CP065338:2778620-2778913 [Klebsiella michiganensis]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEK

Download         Length: 294 bp

>AT182358 NZ_CP086423:342974-343195 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q8N8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q8I3

References