182357

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2064402..2064949 Replicon chromosome
Accession NZ_CP065338
Organism Klebsiella michiganensis strain LDS17

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag I4W82_RS09590 Protein ID WP_014227299.1
Coordinates 2064402..2064710 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag I4W82_RS09595 Protein ID WP_014227298.1
Coordinates 2064713..2064949 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4W82_RS09565 (I4W82_09560) 2060318..2060629 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
I4W82_RS09570 (I4W82_09565) 2060924..2061865 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
I4W82_RS09575 (I4W82_09570) 2061889..2062182 - 294 WP_014227302.1 YicS family protein -
I4W82_RS09580 (I4W82_09575) 2062392..2063345 + 954 WP_032751828.1 Rpn family recombination-promoting nuclease/putative transposase -
I4W82_RS09585 (I4W82_09580) 2063349..2064251 - 903 WP_014227300.1 EamA family transporter -
I4W82_RS09590 (I4W82_09585) 2064402..2064710 - 309 WP_014227299.1 CcdB family protein Toxin
I4W82_RS09595 (I4W82_09590) 2064713..2064949 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I4W82_RS09600 (I4W82_09595) 2065055..2066488 - 1434 WP_032751833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
I4W82_RS09605 (I4W82_09600) 2066513..2067415 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
I4W82_RS09610 (I4W82_09605) 2067576..2068742 - 1167 WP_085520169.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T182357 WP_014227299.1 NZ_CP065338:c2064710-2064402 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T182357 NZ_CP086419:c21253-20837 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT182357 WP_014227298.1 NZ_CP065338:c2064949-2064713 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT182357 NZ_CP086419:c21480-21250 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References