Detailed information of TA system TA182356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1270255..1270831
• Replicon: chromosome
• Accession: NZ_CP065338
• Organism: Klebsiella michiganensis strain LDS17

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1Q8YXR6
• Locus tag: I4W82_RS05870
• Protein ID: WP_046878034.1
• Coordinates: 1270544..1270831 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: I4W82_RS05865
• Protein ID: WP_209420206.1
• Coordinates: 1270255..1270557 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I4W82_RS05845 (I4W82_05845)	1265387..1266736	+	1350	WP_209420204.1	restriction endonuclease subunit S	-
I4W82_RS05850 (I4W82_05850)	1267086..1267421	+	336	WP_032747749.1	endoribonuclease SymE	-
I4W82_RS05855 (I4W82_05855)	1267883..1268794	+	912	WP_032747748.1	acetamidase/formamidase family protein	-
I4W82_RS05860 (I4W82_05860)	1268791..1270134	+	1344	WP_209420205.1	APC family permease	-
I4W82_RS05865 (I4W82_05865)	1270255..1270557	-	303	WP_209420206.1	BrnA antitoxin family protein	Antitoxin
I4W82_RS05870 (I4W82_05870)	1270544..1270831	-	288	WP_046878034.1	BrnT family toxin	Toxin
I4W82_RS05875 (I4W82_05875)	1270969..1271412	-	444	WP_014227755.1	FosA family fosfomycin resistance glutathione transferase	-
I4W82_RS05880 (I4W82_05880)	1271406..1272314	-	909	WP_032747747.1	LysR family transcriptional regulator	-
I4W82_RS05885 (I4W82_05885)	1272402..1273184	+	783	WP_064358753.1	NAD(P)H-dependent oxidoreductase	-
I4W82_RS05890 (I4W82_05890)	1273332..1273916	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
I4W82_RS05895 (I4W82_05895)	1273934..1274734	+	801	WP_046878031.1	winged helix-turn-helix domain-containing protein	-
I4W82_RS05900 (I4W82_05900)	1274731..1275249	+	519	WP_064380989.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.69 Da
• Isoelectric Point: 7.4687

>T182356 WP_046878034.1 NZ_CP065338:c1270831-1270544 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T182356 NZ_CP086418:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT182356 WP_209420206.1 NZ_CP065338:c1270557-1270255 [Klebsiella michiganensis]
MNMVKHKRGNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT182356 NZ_CP086418:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A1Q8YXR6