Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 569002..569621 Replicon chromosome
Accession NZ_CP065338
Organism Klebsiella michiganensis strain LDS17

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag I4W82_RS02640 Protein ID WP_004099646.1
Coordinates 569403..569621 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag I4W82_RS02635 Protein ID WP_025107145.1
Coordinates 569002..569376 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4W82_RS02625 (I4W82_02625) 564158..565351 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I4W82_RS02630 (I4W82_02630) 565374..568520 + 3147 WP_032748290.1 multidrug efflux RND transporter permease subunit AcrB -
I4W82_RS02635 (I4W82_02635) 569002..569376 + 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
I4W82_RS02640 (I4W82_02640) 569403..569621 + 219 WP_004099646.1 HHA domain-containing protein Toxin
I4W82_RS02645 (I4W82_02645) 569784..570350 + 567 WP_064379678.1 maltose O-acetyltransferase -
I4W82_RS27040 570322..570456 - 135 WP_223226764.1 hypothetical protein -
I4W82_RS02650 (I4W82_02650) 570477..570947 + 471 WP_014228205.1 YlaC family protein -
I4W82_RS02655 (I4W82_02655) 570922..572376 - 1455 WP_032748288.1 PLP-dependent aminotransferase family protein -
I4W82_RS02660 (I4W82_02660) 572478..573176 + 699 WP_032748285.1 GNAT family protein -
I4W82_RS02665 (I4W82_02665) 573173..573313 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
I4W82_RS02670 (I4W82_02670) 573313..573576 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T182354 WP_004099646.1 NZ_CP065338:569403-569621 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T182354 NZ_CP086416:c5386791-5386408 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT182354 WP_025107145.1 NZ_CP065338:569002-569376 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT182354 NZ_CP086416:c5387033-5386791 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References