Detailed information of TA system TA182352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-tacA/GNAT-DUF1778
• Location: 2086565..2087328
• Replicon: chromosome
• Accession: NZ_CP065269
• Organism: Mycobacteroides abscessus subsp. abscessus strain GD69A

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: U5X3E3
• Locus tag: I3U40_RS10415
• Protein ID: WP_023363547.1
• Coordinates: 2086565..2087062 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: I3U40_RS10420
• Protein ID: WP_053082894.1
• Coordinates: 2087059..2087328 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U40_RS10395	2083176..2083808	+	633	WP_023363553.1	class I SAM-dependent methyltransferase	-
I3U40_RS10400	2083998..2084594	-	597	WP_023363551.1	ABATE domain-containing protein	-
I3U40_RS10405	2084695..2085264	+	570	WP_023363549.1	TIGR03086 family protein	-
I3U40_RS10410	2085300..2086169	+	870	WP_052622775.1	pyridoxamine 5'-phosphate oxidase family protein	-
I3U40_RS10415	2086565..2087062	-	498	WP_023363547.1	GNAT family N-acetyltransferase	Toxin
I3U40_RS10420	2087059..2087328	-	270	WP_053082894.1	DUF1778 domain-containing protein	Antitoxin
I3U40_RS10425	2087491..2088747	+	1257	WP_005118792.1	beta-ketoacyl synthase	-
I3U40_RS10430	2088787..2089557	+	771	WP_005080193.1	3-oxoacyl-ACP reductase FabG	-
I3U40_RS10435	2089654..2090403	+	750	WP_005114250.1	thioesterase II family protein	-
I3U40_RS10440	2090464..2091405	+	942	WP_005080188.1	ACP S-malonyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-82)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18258.94 Da
• Isoelectric Point: 9.5209

>T182352 WP_023363547.1 NZ_CP065269:c2087062-2086565 [Mycobacteroides abscessus subsp. abscessus]
VSYEGPVLFGADHIVDDFDCGNDVLNEWLRTKALHNQREGGSRTWVVLDGRRVVAFYASSTAVLMRSRATKRAARNQPDP
LPAVLLGRLAVDANHQGRGLAAALLKHFILKSLEVAELTGVRLLLVHANNDTARRFYLRYGFEPSPVDDLTLMLLVKDVR
PSPSA

Nucleotide

• Length: 498 bp

>T182352 NZ_CP086416:c4788059-4787526 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTTGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9925.25 Da
• Isoelectric Point: 5.6845

>AT182352 WP_053082894.1 NZ_CP065269:c2087328-2087059 [Mycobacteroides abscessus subsp. abscessus]
VETKSGRWHLRVTAAQDAVVRRVLDVTGESLNDYVVRHAVQAAEADLADRRVFVLDDAAWTDLQALLDRPPSPKPELARL
LANPSILER

Nucleotide

• Length: 270 bp

>AT182352 NZ_CP086416:c4788336-4788070 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A8B4DM98