Detailed information of TA system TA182351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-tacA/GNAT-DUF1778
• Location: 2086566..2087329
• Replicon: chromosome
• Accession: NZ_CP065266
• Organism: Mycobacteroides abscessus subsp. abscessus strain GD69B

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: U5X3E3
• Locus tag: I3U31_RS10420
• Protein ID: WP_023363547.1
• Coordinates: 2086566..2087063 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: I3U31_RS10425
• Protein ID: WP_053082894.1
• Coordinates: 2087060..2087329 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U31_RS10400	2083177..2083809	+	633	WP_023363553.1	class I SAM-dependent methyltransferase	-
I3U31_RS10405	2083999..2084595	-	597	WP_023363551.1	ABATE domain-containing protein	-
I3U31_RS10410	2084696..2085265	+	570	WP_023363549.1	TIGR03086 family protein	-
I3U31_RS10415	2085301..2086170	+	870	WP_052622775.1	pyridoxamine 5'-phosphate oxidase family protein	-
I3U31_RS10420	2086566..2087063	-	498	WP_023363547.1	GNAT family N-acetyltransferase	Toxin
I3U31_RS10425	2087060..2087329	-	270	WP_053082894.1	DUF1778 domain-containing protein	Antitoxin
I3U31_RS10430	2087492..2088748	+	1257	WP_005118792.1	beta-ketoacyl synthase	-
I3U31_RS10435	2088788..2089558	+	771	WP_005080193.1	3-oxoacyl-ACP reductase FabG	-
I3U31_RS10440	2089655..2090404	+	750	WP_005114250.1	thioesterase II family protein	-
I3U31_RS10445	2090465..2091406	+	942	WP_005080188.1	ACP S-malonyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (9-82)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18258.94 Da
• Isoelectric Point: 9.5209

>T182351 WP_023363547.1 NZ_CP065266:c2087063-2086566 [Mycobacteroides abscessus subsp. abscessus]
VSYEGPVLFGADHIVDDFDCGNDVLNEWLRTKALHNQREGGSRTWVVLDGRRVVAFYASSTAVLMRSRATKRAARNQPDP
LPAVLLGRLAVDANHQGRGLAAALLKHFILKSLEVAELTGVRLLLVHANNDTARRFYLRYGFEPSPVDDLTLMLLVKDVR
PSPSA

Nucleotide

• Length: 498 bp

>T182351 NZ_CP086416:4191545-4191763 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9925.25 Da
• Isoelectric Point: 5.6845

>AT182351 WP_053082894.1 NZ_CP065266:c2087329-2087060 [Mycobacteroides abscessus subsp. abscessus]
VETKSGRWHLRVTAAQDAVVRRVLDVTGESLNDYVVRHAVQAAEADLADRRVFVLDDAAWTDLQALLDRPPSPKPELARL
LANPSILER

Nucleotide

• Length: 270 bp

>AT182351 NZ_CP086416:4191144-4191518 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A8B4DM98