Detailed information of TA system TA182350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2419233..2419798
• Replicon: chromosome
• Accession: NZ_CP065261
• Organism: Streptomyces clavuligerus strain F1D31

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: I3J17_RS09760
• Protein ID: WP_003960643.1
• Coordinates: 2419233..2419601 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: I3J17_RS09765
• Protein ID: WP_029182939.1
• Coordinates: 2419601..2419798 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3J17_RS09740	2414551..2415021	+	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-
I3J17_RS09745	2415086..2415874	-	789	WP_065757152.1	hypothetical protein	-
I3J17_RS09750	2416339..2416980	-	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
I3J17_RS09755	2417282..2419075	-	1794	WP_003954793.1	DEAD/DEAH box helicase	-
I3J17_RS09760	2419233..2419601	-	369	WP_003960643.1	Fic family protein	Toxin
I3J17_RS09765	2419601..2419798	-	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
I3J17_RS09770	2419832..2421337	-	1506	WP_003954790.1	MFS transporter	-
I3J17_RS09780	2421848..2422411	-	564	WP_003954788.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T182350 WP_003960643.1 NZ_CP065261:c2419601-2419233 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T182350 NZ_CP086416:c4012914-4012597 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT182350 WP_029182939.1 NZ_CP065261:c2419798-2419601 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT182350 NZ_CP086416:c4012604-4012317 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: E2Q583