Detailed information of TA system TA182349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2412943..2413508
• Replicon: chromosome
• Accession: NZ_CP065258
• Organism: Streptomyces clavuligerus strain F1D14

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: I3J08_RS09735
• Protein ID: WP_003960643.1
• Coordinates: 2412943..2413311 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: I3J08_RS09740
• Protein ID: WP_029182939.1
• Coordinates: 2413311..2413508 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3J08_RS09715	2408261..2408731	+	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-
I3J08_RS09720	2408796..2409584	-	789	WP_065757152.1	hypothetical protein	-
I3J08_RS09725	2410049..2410690	-	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
I3J08_RS09730	2410992..2412785	-	1794	WP_003954793.1	DEAD/DEAH box helicase	-
I3J08_RS09735	2412943..2413311	-	369	WP_003960643.1	Fic family protein	Toxin
I3J08_RS09740	2413311..2413508	-	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
I3J08_RS09745	2413542..2415047	-	1506	WP_003954790.1	MFS transporter	-
I3J08_RS09755	2415558..2416121	-	564	WP_003954788.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T182349 WP_003960643.1 NZ_CP065258:c2413311-2412943 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T182349 NZ_CP086416:c2798028-2796706 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCTACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT182349 WP_029182939.1 NZ_CP065258:c2413508-2413311 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT182349 NZ_CP086416:c2798294-2798028 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: E2Q583