Detailed information of TA system TA182347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 4450701..4451266
• Replicon: chromosome
• Accession: NZ_CP065253
• Organism: Streptomyces clavuligerus strain F1D7

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: I3J06_RS18315
• Protein ID: WP_003960643.1
• Coordinates: 4450898..4451266 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: I3J06_RS18310
• Protein ID: WP_029182939.1
• Coordinates: 4450701..4450898 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3J06_RS18295	4448088..4448651	+	564	WP_003954788.1	AAA family ATPase	-
I3J06_RS18305	4449162..4450667	+	1506	WP_003954790.1	MFS transporter	-
I3J06_RS18310	4450701..4450898	+	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
I3J06_RS18315	4450898..4451266	+	369	WP_003960643.1	Fic family protein	Toxin
I3J06_RS18320	4451424..4453217	+	1794	WP_003954793.1	DEAD/DEAH box helicase	-
I3J06_RS18325	4453519..4454160	+	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
I3J06_RS18330	4454625..4455413	+	789	WP_065757152.1	hypothetical protein	-
I3J06_RS18335	4455478..4455948	-	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T182347 WP_003960643.1 NZ_CP065253:4450898-4451266 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T182347 NZ_CP086416:1841824-1842156 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT182347 WP_029182939.1 NZ_CP065253:4450701-4450898 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT182347 NZ_CP086416:1841566-1841823 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: E2Q583