Detailed information of TA system TA182345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 4449241..4449806
• Replicon: chromosome
• Accession: NZ_CP065246
• Organism: Streptomyces clavuligerus strain F1D1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: I3J04_RS18320
• Protein ID: WP_003960643.1
• Coordinates: 4449438..4449806 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: I3J04_RS18315
• Protein ID: WP_029182939.1
• Coordinates: 4449241..4449438 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3J04_RS18300	4446628..4447191	+	564	WP_003954788.1	AAA family ATPase	-
I3J04_RS18310	4447702..4449207	+	1506	WP_003954790.1	MFS transporter	-
I3J04_RS18315	4449241..4449438	+	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
I3J04_RS18320	4449438..4449806	+	369	WP_003960643.1	Fic family protein	Toxin
I3J04_RS18325	4449964..4451757	+	1794	WP_003954793.1	DEAD/DEAH box helicase	-
I3J04_RS18330	4452059..4452700	+	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
I3J04_RS18335	4453165..4453953	+	789	WP_065757152.1	hypothetical protein	-
I3J04_RS18340	4454018..4454488	-	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T182345 WP_003960643.1 NZ_CP065246:4449438-4449806 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T182345 NZ_CP086416:343191-343559 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT182345 WP_029182939.1 NZ_CP065246:4449241-4449438 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT182345 NZ_CP086416:342973-343194 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: E2Q583