Detailed information of TA system TA182343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 4459617..4460182
• Replicon: chromosome
• Accession: NZ_CP065241
• Organism: Streptomyces clavuligerus strain F1D35

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E2Q583
• Locus tag: I3J18_RS18350
• Protein ID: WP_003960643.1
• Coordinates: 4459814..4460182 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: I3J18_RS18345
• Protein ID: WP_029182939.1
• Coordinates: 4459617..4459814 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3J18_RS18330	4457004..4457567	+	564	WP_003954788.1	AAA family ATPase	-
I3J18_RS18340	4458078..4459583	+	1506	WP_003954790.1	MFS transporter	-
I3J18_RS18345	4459617..4459814	+	198	WP_029182939.1	Arc family DNA-binding protein	Antitoxin
I3J18_RS18350	4459814..4460182	+	369	WP_003960643.1	Fic family protein	Toxin
I3J18_RS18355	4460340..4462133	+	1794	WP_003954793.1	DEAD/DEAH box helicase	-
I3J18_RS18360	4462435..4463076	+	642	WP_009996313.1	helix-turn-helix domain-containing protein	-
I3J18_RS18365	4463541..4464329	+	789	WP_065757152.1	hypothetical protein	-
I3J18_RS18370	4464394..4464864	-	471	WP_003954796.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-119)

Antitoxin

• Arc-like DNA binding domain (5-29)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13688.61 Da
• Isoelectric Point: 5.1348

>T182343 WP_003960643.1 NZ_CP065241:4459814-4460182 [Streptomyces clavuligerus]
MHYLTLPELLNLAERLRAAEVRDYGLLDSALARPQASVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HVNGHPLDPEFDVDEAERFVLDVCQGALDVPKIASGLLRFAR

Nucleotide

• Length: 369 bp

>T182343 NZ_CP086414:15258-15602 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7424.32 Da
• Isoelectric Point: 5.7030

>AT182343 WP_029182939.1 NZ_CP065241:4459617-4459814 [Streptomyces clavuligerus]
MAGLNVRFTEEELTALRERAEAEGRSMQSFAHDAVIRAINERARLFNEAAEHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT182343 NZ_CP086414:15001-15264 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: E2Q583