Detailed information of TA system TA182331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3774393..3776140
• Replicon: chromosome
• Accession: NZ_CP065230
• Organism: Halomonas sp. A40-4

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IT895_RS17480
• Protein ID: WP_199284190.1
• Coordinates: 3774393..3775049 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IT895_RS17485
• Protein ID: WP_199284191.1
• Coordinates: 3775046..3776140 (+)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT895_RS17460	3770145..3770390	+	246	WP_199285517.1	helix-turn-helix transcriptional regulator	-
IT895_RS17465	3770409..3772832	+	2424	WP_199284188.1	N-6 DNA methylase	-
IT895_RS17470	3772825..3774081	+	1257	WP_199284189.1	restriction endonuclease subunit S	-
IT895_RS17475	3774139..3774390	+	252	Protein_3412	DUF262 domain-containing protein	-
IT895_RS17480	3774393..3775049	+	657	WP_199284190.1	DUF4433 domain-containing protein	Toxin
IT895_RS17485	3775046..3776140	+	1095	WP_199284191.1	macro domain-containing protein	Antitoxin
IT895_RS17490	3776801..3780169	+	3369	WP_199284192.1	type I restriction endonuclease subunit R	-
IT895_RS17495	3780166..3780867	+	702	WP_199284193.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3772825..3780867	8042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 24587.16 Da
• Isoelectric Point: 8.4957

>T182331 WP_199284190.1 NZ_CP065230:3774393-3775049 [Halomonas sp. A40-4]
VATDLSRSLNSRKALIFRITHRDNLPWLLAHGLYCRSAAEQDPGFVTIGNRDLIGRRATRDVPTPPGGTLSDYIPFYFTP
ASPMLLNIVTGRGVPIQRKEDILVLVCSLPRLQEIGSAFVFTDRHAYLPMARFSSQLVNLEEFVPWPLLQAQDFSRDRED
PEKTERYQAEALAHHHLPASALLGIGTYTDEVRVAVRAQVAAHQLSLKVVTRPNWFFQ

Nucleotide

• Length: 657 bp

>T182331 NZ_CP086410:1347-1652 [Enterobacter cloacae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 41500.75 Da
• Isoelectric Point: 5.7359

>AT182331 WP_199284191.1 NZ_CP065230:3775046-3776140 [Halomonas sp. A40-4]
MIEYTRGNLLEADVEALVNTVNTVGVMGKGIALMFKEAFPENFAAYEAACKHQQVVTGRMFVTERDALLGPRWIINFPTK
QHWRAKTRVEWIESGLEDLKQVIREKGIRSIALPPLGCGNGGLDWAMVRPRIEAALAELDNVRVLVFEPTVEYQNVAKRS
GVERLTPARAMIAELVRRYWVLGIECSLLEVQKLAWLMEQRIIAHGLENPLKLEFQPHRYGPYSDRLRHLLNGLDGSYLH
SERRINDATPEEVVWFDESRRDRLHVYLRSAEARPYVGVVEEVDALIDGFQSPLGMEVLATLDWLISQEYVEPTVEAIKE
GLRYWPDDIAGQRKLRLFSDRLIALALERLREGSPTTQAISTAL

Nucleotide

• Length: 1095 bp

>AT182331 NZ_CP086410:1127-1345 [Enterobacter cloacae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA