Detailed information of TA system TA182323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1100529..1101274
• Replicon: chromosome
• Accession: NZ_CP065228
• Organism: Xanthomonas oryzae pv. oryzae strain BXO512

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: BXO512_RS05065
• Protein ID: WP_205420989.1
• Coordinates: 1100529..1100858 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: BXO512_RS05070
• Protein ID: WP_237384720.1
• Coordinates: 1100855..1101274 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO512_RS05035 (BXO512_004965)	1095659..1096330	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
BXO512_RS05045 (BXO512_004975)	1096633..1097424	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
BXO512_RS05050 (BXO512_004980)	1097470..1098396	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
BXO512_RS05055 (BXO512_004985)	1098728..1099063	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
BXO512_RS05060 (BXO512_004990)	1099107..1100426	-	1320	WP_109182077.1	IS701-like element ISXo15 family transposase	-
BXO512_RS05065 (BXO512_004995)	1100529..1100858	+	330	WP_205420989.1	DUF4433 domain-containing protein	Toxin
BXO512_RS05070 (BXO512_005000)	1100855..1101274	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
BXO512_RS05075 (BXO512_005005)	1101326..1102294	+	969	WP_011258529.1	IS5-like element ISXo1 family transposase	-
BXO512_RS05080 (BXO512_005010)	1102486..1103454	+	969	WP_011258188.1	IS5-like element ISXo1 family transposase	-
BXO512_RS05085 (BXO512_005015)	1103607..1104245	+	639	WP_011407753.1	phosphate phosphatase	-
BXO512_RS05090 (BXO512_005020)	1104283..1105518	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
BXO512_RS05095 (BXO512_005025)	1105820..1106146	+	327	Protein_981	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1099107..1105518	6411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13159.89 Da
• Isoelectric Point: 7.1161

>T182323 WP_205420989.1 NZ_CP065228:1100529-1100858 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSELSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T182323 NZ_CP086408:c1179402-1179184 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGTCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT182323 WP_237384720.1 NZ_CP065228:1100855-1101274 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT182323 NZ_CP086408:c1179804-1179430 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA