Detailed information of TA system TA182322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 201017..201653
• Replicon: chromosome
• Accession: NZ_CP065213
• Organism: Priestia megaterium strain HGS7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: I3X02_RS01130
• Protein ID: WP_013055004.1
• Coordinates: 201303..201653 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: I3X02_RS01125
• Protein ID: WP_013055003.1
• Coordinates: 201017..201298 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3X02_RS01100	196383..197354	+	972	WP_042992385.1	UV DNA damage repair endonuclease UvsE	-
I3X02_RS01105	197363..197965	-	603	WP_014461974.1	rhomboid family intramembrane serine protease	-
I3X02_RS01110	198030..198395	+	366	WP_042992604.1	holo-ACP synthase	-
I3X02_RS01115	198508..199512	+	1005	WP_279400964.1	outer membrane lipoprotein carrier protein LolA	-
I3X02_RS01120	199626..200816	+	1191	WP_014461971.1	alanine racemase	-
I3X02_RS01125	201017..201298	+	282	WP_013055003.1	hypothetical protein	Antitoxin
I3X02_RS01130	201303..201653	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I3X02_RS01135	201811..202647	+	837	WP_014461969.1	RsbT co-antagonist protein RsbRA	-
I3X02_RS01140	202650..203006	+	357	WP_013055006.1	STAS domain-containing protein	-
I3X02_RS01145	203010..203411	+	402	WP_013055007.1	anti-sigma regulatory factor	-
I3X02_RS01150	203425..204435	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
I3X02_RS01155	204495..204827	+	333	WP_014461968.1	anti-sigma factor antagonist	-
I3X02_RS01160	204824..205309	+	486	WP_014461967.1	anti-sigma B factor RsbW	-
I3X02_RS01165	205275..206069	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T182322 WP_013055004.1 NZ_CP065213:201303-201653 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T182322 NZ_CP086408:635010-635297 [Enterobacter cloacae]
ATGCCAACGGAGTATGAGTGGGATAGCGATAAGGCGAAAAGTAACCAGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TCTGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAGTTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCGTCATTGTTATCCTGGTCGCACATACGGTACGTTTCGAAAGCGGAGGCGAAATTATTCGTATCATTAGC
GCACGTAAGGCGGACCGTAAAGAGAGGAACCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT182322 WP_013055003.1 NZ_CP065213:201017-201298 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT182322 NZ_CP086408:635284-635586 [Enterobacter cloacae]
ATGAGCATCGTTAAACATAAACGAGGTAGTTCGCCTGAGGTTAGCGCTCGTCGTGACGCTGAGCTAAAAGCATTAGCTGA
AAAACCAGAAGCAGAAATCGATTACAGCGATATTCCTGCCACGGACGATAAACAGTGGTCCGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTTAAAACGCAGGCATCTGTACGCATTGACGCCGACGTGATGGAGTGGCTAAAACGGCCTGGAAAGGGT
TACCAGACGCGGCTAAACGCCATATTGCGTGAAGCGATGCTGCGCGATCAAAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9