182321

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 385273..385844 Replicon chromosome
Accession NZ_CP065211
Organism Enterococcus lactis strain KCTC 21015

Toxin (Protein)


Gene name mazF Uniprot ID A0A2W4HYX0
Locus tag I4Q40_RS01870 Protein ID WP_002315988.1
Coordinates 385503..385844 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag I4Q40_RS01865 Protein ID WP_025477367.1
Coordinates 385273..385503 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4Q40_RS01845 381537..382130 + 594 WP_002315992.1 malonate decarboxylase holo-ACP synthase -
I4Q40_RS01850 382154..383239 + 1086 WP_002329651.1 acyltransferase -
I4Q40_RS01855 383261..384130 + 870 WP_002315990.1 triphosphoribosyl-dephospho-CoA synthase MdcB -
I4Q40_RS01860 384242..385006 + 765 WP_002292530.1 TIM44-like domain-containing protein -
I4Q40_RS01865 385273..385503 + 231 WP_025477367.1 hypothetical protein Antitoxin
I4Q40_RS01870 385503..385844 + 342 WP_002315988.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I4Q40_RS01875 386539..388620 + 2082 WP_156272237.1 BglG family transcription antiterminator -
I4Q40_RS01880 388656..388958 + 303 WP_002292534.1 PTS sugar transporter subunit IIB -
I4Q40_RS01885 388984..390273 + 1290 WP_002315985.1 PTS sugar transporter subunit IIC -
I4Q40_RS01890 390292..390699 + 408 WP_002315984.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13330.69 Da        Isoelectric Point: 9.9258

>T182321 WP_002315988.1 NZ_CP065211:385503-385844 [Enterococcus lactis]
VSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T182321 NZ_CP086408:c628899-628378 [Enterobacter cloacae]
GTGAATAACATGAAAATAGGGATTTTTTCAGATGACGTTGAGTATGATCTAAGCCATTTCGATTGCGGAGAAGAGAGCCT
AAACACCTTTCTGACGGCGCACCTGAAACGCCAGCATCGTGGGAAATTTTTGCGGGGTTACGTTCTTGTCGCATCCGGGG
AAAAGCCGCGGGTGTTGGGTTATTACACGCTGTCCGGGAGCTGCTTTGAAAAGGCGTACCTTCCTTCTAAAACCCAGCAG
AAGCGAGTGCCTTACAAAAATGTTCCCAGCGTCACGCTGGGCCGCCTGGCGATAGATAAACGCTTCCAGGGGCAAGGATT
GGGGGAGTTGCTGGTCACCCATGCTCTGAAAACGGTCTACCTGGCGTCTTTTGCCGTGGGTATCCACGGGATATTCGTAG
AAGCGCTGAACGACAGCGCCAAAAACTTTTACCTTAAACTGGGGTTTATCGCCCTTCAGGCAGAGAATGAAAATACTCTC
TTCCTGCCGACTAAAACCATCGAAAGATTGTTTGCAGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT182321 WP_025477367.1 NZ_CP065211:385273-385503 [Enterococcus lactis]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT182321 NZ_CP086408:c629172-628903 [Enterobacter cloacae]
ATGTCAGCACTAAAGAAACAGCGCATCGATCTACGCTTAACCGAAGACGACAAAAGCCTGATCGAAGAAGCAGCGGCAAT
GACGAACCAGTCAATTTCACAATTCATGGTCAGTACGGCCTCCGAACGCGCAGCAGAAGTGATTGAACAGCACCGCCGCC
TGATCCTCAACGAGGCATCATGGAACCAGGTGATGGATGCGATCAGTAACCCGCCAACGCCAAATGAACGACTGAAGCGA
GCAGCCAGGCGTCTCCAGGATCTGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W4HYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References