Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 216090..216619 Replicon chromosome
Accession NZ_CP065211
Organism Enterococcus lactis strain KCTC 21015

Toxin (Protein)


Gene name relE Uniprot ID C9B8W9
Locus tag I4Q40_RS01070 Protein ID WP_002294198.1
Coordinates 216353..216619 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C9B8W8
Locus tag I4Q40_RS01065 Protein ID WP_002294199.1
Coordinates 216090..216353 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4Q40_RS01050 212496..213941 + 1446 WP_070703901.1 alpha-N-arabinofuranosidase -
I4Q40_RS01055 213946..214212 + 267 WP_002315578.1 hypothetical protein -
I4Q40_RS01060 214265..215455 + 1191 WP_002315579.1 arabinan endo-1,5-alpha-L-arabinosidase -
I4Q40_RS01065 216090..216353 + 264 WP_002294199.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I4Q40_RS01070 216353..216619 + 267 WP_002294198.1 Txe/YoeB family addiction module toxin Toxin
I4Q40_RS01075 216815..217009 + 195 WP_080000635.1 hypothetical protein -
I4Q40_RS01080 217141..217386 + 246 WP_127808464.1 hypothetical protein -
I4Q40_RS01085 217367..217570 + 204 WP_002315581.1 glycosyl hydrolase family 85 -
I4Q40_RS01090 217889..218560 - 672 WP_025477296.1 ABC transporter permease -
I4Q40_RS01095 218557..219474 - 918 WP_002315583.1 osmoprotectant ABC transporter substrate-binding protein -
I4Q40_RS01100 219471..220112 - 642 WP_002293405.1 ABC transporter permease -
I4Q40_RS01105 220116..221291 - 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 221402..222361 959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(22-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10646.11 Da        Isoelectric Point: 9.9931

>T182320 WP_002294198.1 NZ_CP065211:216353-216619 [Enterococcus lactis]
MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T182320 NZ_CP086408:538409-538693 [Enterobacter cloacae]
ATGACTTATGAACTGGCATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTAGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCAGCCCGTCTGCATAATCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCTATTGGGAAA
AGAGAGAAATTAACCGTGTATCACGATGCTAACAAACGGCTATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10016.03 Da        Isoelectric Point: 4.1207

>AT182320 WP_002294199.1 NZ_CP065211:216090-216353 [Enterococcus lactis]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Download         Length: 264 bp

>AT182320 NZ_CP086408:538177-538419 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGACGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FMA0

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZU84

References