Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 478196..478767 Replicon chromosome
Accession NZ_CP065210
Organism Enterococcus faecium strain KCCM 12118

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag I4Q37_RS02270 Protein ID WP_002286801.1
Coordinates 478426..478767 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag I4Q37_RS02265 Protein ID WP_002323011.1
Coordinates 478196..478426 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4Q37_RS02240 473439..474770 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
I4Q37_RS02245 474792..475418 + 627 WP_002333420.1 cysteine hydrolase -
I4Q37_RS02250 475601..476182 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
I4Q37_RS02255 476791..477366 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
I4Q37_RS02260 477571..477909 - 339 WP_002286804.1 hypothetical protein -
I4Q37_RS02265 478196..478426 + 231 WP_002323011.1 hypothetical protein Antitoxin
I4Q37_RS02270 478426..478767 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I4Q37_RS02275 479617..479802 + 186 WP_002304207.1 hypothetical protein -
I4Q37_RS02280 480307..480501 + 195 WP_002295146.1 hypothetical protein -
I4Q37_RS02285 480623..480941 + 319 Protein_453 transposase -
I4Q37_RS02290 481185..482015 - 831 WP_002286789.1 manganese catalase family protein -
I4Q37_RS02295 482223..483557 + 1335 WP_002333419.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T182319 WP_002286801.1 NZ_CP065210:478426-478767 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T182319 NZ_CP086408:c284007-283705 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACTGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCAAGGACCATTATCGC
GTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACATAAAACCCGTGGGGGAAGGTATATGCGAGCTGAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT182319 WP_002323011.1 NZ_CP065210:478196-478426 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT182319 NZ_CP086408:c283698-283411 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCATCGCGTTGGTAGATGATGAGGAAATAGCCGTGTTTATGGCTGATGCATT
AGAAACCGGTGACTCAGCGTATATTGCTAAAGCTTTGGGCGTCATCGCGCGAGCGAAAGGGATGTCGACCATTTCACAGC
AAACTGGCCTGTCACGGGAGCAACTGTACCGATCATTTAGTGATAAGGGGAACCCAACGCTTAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTCACAATCAAACCCGCCGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References