Detailed information of TA system TA182311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 421827..422431
• Replicon: chromosome
• Accession: NZ_CP065202
• Organism: Pseudomonas fragi strain A13BB

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: I5R27_RS01975
• Protein ID: WP_081481088.1
• Coordinates: 421827..422024 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A494R758
• Locus tag: I5R27_RS01980
• Protein ID: WP_016783077.1
• Coordinates: 422021..422431 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5R27_RS01950	416865..417044	+	180	WP_086799343.1	type II toxin-antitoxin system HicA family toxin	-
I5R27_RS01955	417085..417492	+	408	WP_086799341.1	type II toxin-antitoxin system HicB family antitoxin	-
I5R27_RS01960	417589..420027	-	2439	WP_086799338.1	TonB-dependent siderophore receptor	-
I5R27_RS01965	420140..421087	-	948	WP_196883468.1	FecR domain-containing protein	-
I5R27_RS01970	421087..421587	-	501	WP_086799334.1	sigma-70 family RNA polymerase sigma factor	-
I5R27_RS01975	421827..422024	+	198	WP_081481088.1	type II toxin-antitoxin system HicA family toxin	Toxin
I5R27_RS01980	422021..422431	+	411	WP_016783077.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I5R27_RS01985	422983..423333	+	351	WP_196883470.1	hypothetical protein	-
I5R27_RS01990	423378..424388	+	1011	WP_196883472.1	hypothetical protein	-
I5R27_RS01995	424415..425386	-	972	WP_196883474.1	hypothetical protein	-
I5R27_RS02000	425371..426063	-	693	WP_106117233.1	hypothetical protein	-
I5R27_RS02005	426069..426602	-	534	WP_196883476.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	420140..456601	36461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7226.44 Da
• Isoelectric Point: 11.0468

>T182311 WP_081481088.1 NZ_CP065202:421827-422024 [Pseudomonas fragi]
MRSRELIELLMADGWFEIAVKGSHHQFKHPAKAGRVTVPHPRTEIAKGTLHSILKSAGLKGELRS

Nucleotide

• Length: 198 bp

>T182311 NZ_CP086405:1677728-1678249 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTAGCATCGCAGGCGGTGGGTGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGGTGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14844.63 Da
• Isoelectric Point: 4.4720

>AT182311 WP_016783077.1 NZ_CP065202:422021-422431 [Pseudomonas fragi]
MKFPVVIHKDADSEFSVTVPDVPGCFSAGATFSQALDNVQEALALHLEGIVADGGELPQGKDVDAHLENPDYVGGIWAIV
DFDLTPYLGKSVRFNASLPENLLQRIDERVSRDHRYASRSGFLATAALRELSVQNF

Nucleotide

• Length: 411 bp

>AT182311 NZ_CP086405:1677452-1677721 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A494R758