Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2092159..2092673 Replicon chromosome
Accession NZ_CP065199
Organism Staphylococcus aureus strain DG36

Toxin (Protein)


Gene name TsdT Uniprot ID A0A641A3V2
Locus tag I3K83_RS10180 Protein ID WP_000581793.1
Coordinates 2092159..2092368 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag I3K83_RS10185 Protein ID WP_000138932.1
Coordinates 2092380..2092673 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K83_RS10160 2087668..2089026 - 1359 WP_000611482.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
I3K83_RS10165 2089041..2090111 - 1071 WP_000159627.1 D-alanine--D-alanine ligase -
I3K83_RS10170 2090429..2091631 + 1203 WP_031590208.1 rod shape-determining protein RodA -
I3K83_RS10175 2091894..2092031 - 138 WP_000828354.1 hypothetical protein -
I3K83_RS10180 2092159..2092368 - 210 WP_000581793.1 heavy-metal-associated domain-containing protein Toxin
I3K83_RS10185 2092380..2092673 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
I3K83_RS10190 2092839..2094323 + 1485 WP_000571549.1 cardiolipin synthase -
I3K83_RS10195 2094351..2094998 + 648 WP_031590210.1 HD domain-containing protein -
I3K83_RS10200 2095611..2096483 - 873 WP_000725802.1 membrane protein insertase YidC -
I3K83_RS10205 2096569..2097210 - 642 WP_000483155.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7809.75 Da        Isoelectric Point: 4.1008

>T182303 WP_000581793.1 NZ_CP065199:c2092368-2092159 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVI

Download         Length: 210 bp

>T182303 NZ_CP086391:c4251683-4251372 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT182303 WP_000138932.1 NZ_CP065199:c2092673-2092380 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT182303 NZ_CP086391:c4251371-4251081 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A641A3V2

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References