Detailed information of TA system TA182302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2072419..2072948
• Replicon: chromosome
• Accession: NZ_CP065199
• Organism: Staphylococcus aureus strain DG36

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I3K83_RS10090
• Protein ID: WP_000621175.1
• Coordinates: 2072419..2072781 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I3K83_RS10095
• Protein ID: WP_000948331.1
• Coordinates: 2072778..2072948 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K83_RS10070	2069398..2070168	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
I3K83_RS10075	2070143..2070622	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
I3K83_RS10080	2070624..2070950	-	327	WP_001052491.1	anti-sigma factor antagonist	-
I3K83_RS10085	2071069..2072070	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I3K83_RS10090	2072419..2072781	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I3K83_RS10095	2072778..2072948	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I3K83_RS10100	2073033..2074181	-	1149	WP_001281154.1	alanine racemase	-
I3K83_RS10105	2074247..2074606	-	360	WP_000581197.1	holo-ACP synthase	-
I3K83_RS10110	2074610..2075101	-	492	WP_072353916.1	PH domain-containing protein	-
I3K83_RS10115	2075088..2076671	-	1584	WP_001294626.1	PH domain-containing protein	-
I3K83_RS10120	2076664..2077143	-	480	WP_031590204.1	hypothetical protein	-
I3K83_RS10125	2077352..2077912	-	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T182302 WP_000621175.1 NZ_CP065199:c2072781-2072419 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T182302 NZ_CP086391:c3954910-3954737 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT182302 WP_000948331.1 NZ_CP065199:c2072948-2072778 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT182302 NZ_CP086391:c3955234-3954938 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1