Detailed information of TA system TA182301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1906175..1906951
• Replicon: chromosome
• Accession: NZ_CP065199
• Organism: Staphylococcus aureus strain DG36

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: I3K83_RS09135
• Protein ID: WP_000031108.1
• Coordinates: 1906175..1906327 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: I3K83_RS09140
• Protein ID: WP_001251230.1
• Coordinates: 1906352..1906951 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3K83_RS09120	1902263..1903084	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
I3K83_RS09125	1903536..1904921	-	1386	WP_000116229.1	class II fumarate hydratase	-
I3K83_RS09130	1905117..1905512	-	396	WP_196838011.1	hypothetical protein	-
I3K83_RS09135	1906175..1906327	-	153	WP_000031108.1	hypothetical protein	Toxin
I3K83_RS09140	1906352..1906951	-	600	WP_001251230.1	hypothetical protein	Antitoxin
I3K83_RS09145	1907110..1907580	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
I3K83_RS09150	1907585..1908712	-	1128	WP_031590038.1	tRNA epoxyqueuosine(34) reductase QueG	-
I3K83_RS09155	1908863..1909585	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
I3K83_RS09160	1909578..1911035	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T182301 WP_000031108.1 NZ_CP065199:c1906327-1906175 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T182301 NZ_CP086391:c3851298-3850948 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCTATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22369.51 Da
• Isoelectric Point: 4.9728

>AT182301 WP_001251230.1 NZ_CP065199:c1906951-1906352 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKYAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT182301 NZ_CP086391:c3851543-3851292 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: X5E2E6